Nonenzymatic determination of glucose at near neutral pH values based on the use of nafion and platinum black coated microneedle electrode array

Abstract

The authors report on a microneedle-based amperometric nonenzymatic glucose sensor for painless and continuous monitoring of glucose. It consists of 3 x 5 sharp stainless steel microneedles micromachined from a stainless steel substrate. The microneedles are 600 and 100 mu m in height and width, respectively. Nafion and platinum black were sequentially coated onto the tip of gold-coated microneedles and used for nonenzymatic (direct) sensing of glucose. Attractive features of the modified microneedle electrode include (a) a low working potential (+0.12 V vs. Ag/AgCl), (b) a linear response in the physiologically relevant range (1-40 mM), (c) a sensitivity as high as 175 mu A mM(-1) cm(-2), (d) a 23 mu M detection limit, and (e) a response time of 2 s. The sensor also exhibits good reproducibility and stability. The sensor is selective for glucose even in the presence of 10-fold higher concentrations of ascorbic acid, lactic acid, dopamine, uric acid, and acetaminophen.