A rapid bio-optical sensor for diagnosing Q fever in clinical specimens
DC Field | Value | Language |
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dc.contributor.author | Koo, Bonhan | - |
dc.contributor.author | Jin, Choong Eun | - |
dc.contributor.author | Park, Se Yoon | - |
dc.contributor.author | Lee, Tae Yoon | - |
dc.contributor.author | Nam, Jeonghun | - |
dc.contributor.author | Jang, Young-Rock | - |
dc.contributor.author | Kim, Sun Mi | - |
dc.contributor.author | Kim, Ji Yeun | - |
dc.contributor.author | Kim, Sung-Han | - |
dc.contributor.author | Shin, Yong | - |
dc.date.available | 2020-02-27T11:41:25Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 1864-063X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3932 | - |
dc.description.abstract | Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio-optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio-optical sensor, that transduces the presence of the target DNA based on binding-induced changes in the refractive index on the waveguide surface in a label-free and real-time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20min), 1-step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.relation.isPartOf | JOURNAL OF BIOPHOTONICS | - |
dc.subject | RECOMBINASE POLYMERASE AMPLIFICATION | - |
dc.subject | SILICON MICRORING RESONATORS | - |
dc.subject | COXIELLA-BURNETII | - |
dc.subject | LABEL-FREE | - |
dc.subject | BLADDER-CANCER | - |
dc.subject | UNKNOWN ORIGIN | - |
dc.subject | DNA | - |
dc.subject | PCR | - |
dc.subject | EXTRACTION | - |
dc.subject | BIOSENSOR | - |
dc.title | A rapid bio-optical sensor for diagnosing Q fever in clinical specimens | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000430178100011 | - |
dc.identifier.doi | 10.1002/jbio.201700167 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOPHOTONICS, v.11, no.4 | - |
dc.identifier.scopusid | 2-s2.0-85041139569 | - |
dc.citation.title | JOURNAL OF BIOPHOTONICS | - |
dc.citation.volume | 11 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Jang, Young-Rock | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | bio-optical sensor | - |
dc.subject.keywordAuthor | clinical specimens | - |
dc.subject.keywordAuthor | isothermal DNA amplification | - |
dc.subject.keywordAuthor | Q fever | - |
dc.subject.keywordAuthor | zoonosis | - |
dc.subject.keywordPlus | RECOMBINASE POLYMERASE AMPLIFICATION | - |
dc.subject.keywordPlus | SILICON MICRORING RESONATORS | - |
dc.subject.keywordPlus | COXIELLA-BURNETII | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | BLADDER-CANCER | - |
dc.subject.keywordPlus | UNKNOWN ORIGIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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