A rapid bio-optical sensor for diagnosing Q fever in clinical specimens
- Authors
- Koo, Bonhan; Jin, Choong Eun; Park, Se Yoon; Lee, Tae Yoon; Nam, Jeonghun; Jang, Young-Rock; Kim, Sun Mi; Kim, Ji Yeun; Kim, Sung-Han; Shin, Yong
- Issue Date
- Apr-2018
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- bio-optical sensor; clinical specimens; isothermal DNA amplification; Q fever; zoonosis
- Citation
- JOURNAL OF BIOPHOTONICS, v.11, no.4
- Journal Title
- JOURNAL OF BIOPHOTONICS
- Volume
- 11
- Number
- 4
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3932
- DOI
- 10.1002/jbio.201700167
- ISSN
- 1864-063X
- Abstract
- Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio-optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio-optical sensor, that transduces the presence of the target DNA based on binding-induced changes in the refractive index on the waveguide surface in a label-free and real-time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20min), 1-step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin.
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