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A novel paper-plastic hybrid device for the simultaneous loop-mediated isothermal amplification and detection of DNA
- Authors
- Kim, Jae-Heon; Yoo, In Sang; An, Jeong Ho; Kim, Sanghyo
- Issue Date
- 1-Mar-2018
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Paper-based analytical device; Loop-mediated isothermal amplification; Colorimetric detection; Staphylococcus aureus
- Citation
- MATERIALS LETTERS, v.214, pp.243 - 246
- Journal Title
- MATERIALS LETTERS
- Volume
- 214
- Start Page
- 243
- End Page
- 246
- ISSN
- 0167-577X
- Abstract
- In this study, polyethersulfone (PES) paper embedded with a polymethyl methacrylate (PMMA) platform was developed for simultaneous DNA amplification and colorimetric detection. DNA was amplified using loop-mediated isothermal amplification (LAMP) in isothermal conditions in the presence of the common magnesium-dependent colorimetric indicator, eriochrome black T (EBT), to detect DNA. Here, the DNA of Staphylococcus aureus was used as the DNA template. The developed device was successfully used to simultaneously amplify and detect the DNA by the naked eye within 30 min with a detection limit of 1 fg/mu L. (C) 2017 Elsevier B.V. All rights reserved.
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Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 공과대학 > 화공생명공학과 > 1. Journal Articles
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Related Researcher
- Yoo, In Sang
- Engineering (Department of Chemical and Biological Engineering)