Immune-enhancing activity of C-militaris fermented with Pediococcus pentosaceus (GRC-ON89A) in CY-induced immunosuppressed model
DC Field | Value | Language |
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dc.contributor.author | Kwon, Ha-Kyoung | - |
dc.contributor.author | Jo, Woo-Ri | - |
dc.contributor.author | Park, Hye-Jin | - |
dc.date.available | 2020-02-27T11:42:17Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-02-23 | - |
dc.identifier.issn | 1472-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4050 | - |
dc.description.abstract | Background: Cordyceps militaris (C. militaris) is reported to exert various immune-activities. To enhance its activity, we fermented C. militaris with Pediococcus pentosaceus ON89A (GRC-ON89A). In this study, we investigated the immune-enhancing activity GRC-ON89A, using immunosuppressed model. Methods: Immunosuppression was induced by intraperitoneal injection of cyclophosphamide (CY). Each group was orally administered distilled water, GRC-ON89A or GRC, respectively. The phagocytic activities against IgG-opsonized FITC particles were measured using phagocytosis assay kit. The contents beta-glucan, cordycepin and SCFA were measured using beta-glucan kit, liquid chromatography-mass spectrometry analysis and Gas chromatography-mass spectrometry analysis, respectively. Results: Among GRC fermented with different probiotic strains (Pediococcus pentossaceus ON89A, Lactobacillus pentosus SC64, Weissella cibaria Sal.Cla22), GRC-ON89A induced the highest elevation of nitric oxide production and enhanced phagocytic activity of RAW 264.7 cells. In primary cultured murine macrophages from normal and CY-treated mice, GRC-ON89A increased phagocytic activity, compared to that in control cells. GRC-ON89A also significantly induced the mRNA expression of TNF-alpha and IL-10 and the levels of phosphorylated Lyn, Syk and MAPK. The contents of beta-glucan, cordycepin and SCFA in GRC significantly increased after ON89A fermentation, compared to those in unfermented GRC. Conclusion: These results indicate that GRC-ON89A exerted the enhanced immunostimulatory activity and contained more nutritional components, compared to unfermented GRC. Our results suggested that GRC-ON89A may be applied as an agent for immune boosting therapy in immune suppressed patients. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.relation.isPartOf | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.subject | CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION | - |
dc.subject | RAW 264.7 MACROPHAGES | - |
dc.subject | CORDYCEPS-MILITARIS | - |
dc.subject | ACIDIC POLYSACCHARIDE | - |
dc.subject | GERMINATED SOYBEANS | - |
dc.subject | CANCER CELLS | - |
dc.subject | LACTIC-ACID | - |
dc.subject | ACTIVATION | - |
dc.subject | MICE | - |
dc.subject | PHAGOCYTOSIS | - |
dc.title | Immune-enhancing activity of C-militaris fermented with Pediococcus pentosaceus (GRC-ON89A) in CY-induced immunosuppressed model | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000426378200001 | - |
dc.identifier.doi | 10.1186/s12906-018-2133-9 | - |
dc.identifier.bibliographicCitation | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.18 | - |
dc.identifier.scopusid | 2-s2.0-85042407167 | - |
dc.citation.title | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.citation.volume | 18 | - |
dc.contributor.affiliatedAuthor | Kwon, Ha-Kyoung | - |
dc.contributor.affiliatedAuthor | Jo, Woo-Ri | - |
dc.contributor.affiliatedAuthor | Park, Hye-Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cordyceps militaris | - |
dc.subject.keywordAuthor | Probiotic fermentation | - |
dc.subject.keywordAuthor | Immune enhancing | - |
dc.subject.keywordAuthor | Pediococcus pentosaceus | - |
dc.subject.keywordAuthor | Cyclophosphamide-induced immune suppression | - |
dc.subject.keywordAuthor | macrophage | - |
dc.subject.keywordPlus | CYCLOPHOSPHAMIDE-INDUCED IMMUNOSUPPRESSION | - |
dc.subject.keywordPlus | RAW 264.7 MACROPHAGES | - |
dc.subject.keywordPlus | CORDYCEPS-MILITARIS | - |
dc.subject.keywordPlus | ACIDIC POLYSACCHARIDE | - |
dc.subject.keywordPlus | GERMINATED SOYBEANS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | LACTIC-ACID | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | PHAGOCYTOSIS | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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