Immune-enhancing activity of C-militaris fermented with Pediococcus pentosaceus (GRC-ON89A) in CY-induced immunosuppressed model
- Authors
- Kwon, Ha-Kyoung; Jo, Woo-Ri; Park, Hye-Jin
- Issue Date
- 23-Feb-2018
- Publisher
- BIOMED CENTRAL LTD
- Keywords
- Cordyceps militaris; Probiotic fermentation; Immune enhancing; Pediococcus pentosaceus; Cyclophosphamide-induced immune suppression; macrophage
- Citation
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.18
- Journal Title
- BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Volume
- 18
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4050
- DOI
- 10.1186/s12906-018-2133-9
- ISSN
- 1472-6882
- Abstract
- Background: Cordyceps militaris (C. militaris) is reported to exert various immune-activities. To enhance its activity, we fermented C. militaris with Pediococcus pentosaceus ON89A (GRC-ON89A). In this study, we investigated the immune-enhancing activity GRC-ON89A, using immunosuppressed model. Methods: Immunosuppression was induced by intraperitoneal injection of cyclophosphamide (CY). Each group was orally administered distilled water, GRC-ON89A or GRC, respectively. The phagocytic activities against IgG-opsonized FITC particles were measured using phagocytosis assay kit. The contents beta-glucan, cordycepin and SCFA were measured using beta-glucan kit, liquid chromatography-mass spectrometry analysis and Gas chromatography-mass spectrometry analysis, respectively. Results: Among GRC fermented with different probiotic strains (Pediococcus pentossaceus ON89A, Lactobacillus pentosus SC64, Weissella cibaria Sal.Cla22), GRC-ON89A induced the highest elevation of nitric oxide production and enhanced phagocytic activity of RAW 264.7 cells. In primary cultured murine macrophages from normal and CY-treated mice, GRC-ON89A increased phagocytic activity, compared to that in control cells. GRC-ON89A also significantly induced the mRNA expression of TNF-alpha and IL-10 and the levels of phosphorylated Lyn, Syk and MAPK. The contents of beta-glucan, cordycepin and SCFA in GRC significantly increased after ON89A fermentation, compared to those in unfermented GRC. Conclusion: These results indicate that GRC-ON89A exerted the enhanced immunostimulatory activity and contained more nutritional components, compared to unfermented GRC. Our results suggested that GRC-ON89A may be applied as an agent for immune boosting therapy in immune suppressed patients.
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