Bee venom phospholipase A2 induces regulatory T cell populations by suppressing apoptotic signaling pathway
- Authors
- Baek H.; Park S.-Y.; Ku S.J.; Ryu K.; Kim Y.; Bae H.; Lee Y.-S.
- Issue Date
- Mar-2020
- Publisher
- MDPI AG
- Keywords
- Apoptosis; Bee venom phospholipase A2; BvPLA2; Regulatory T cells; Tregs
- Citation
- Toxins, v.12, no.3
- Journal Title
- Toxins
- Volume
- 12
- Number
- 3
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/41605
- DOI
- 10.3390/toxins12030198
- ISSN
- 2072-6651
- Abstract
- Bee venom phospholipase A2 is a lipolytic enzyme in bee venom that catalyzes hydrolysis of the sn-2 ester bond of membrane phospholipids to produce free fatty acid and lysophospholipids. Current evidence suggests that bee venom phospholipase A2 (bvPLA2) induces regulatory T cell expansion and attenuates several immune system-related diseases, including Alzheimer’s disease. The induction of Treg cells is directly mediated by binding to mannose receptors on dendritic cells. This interaction induces the PGE2-EP2 signaling pathway, which promotes Treg induction in CD4+ T cells. In this study, we investigated the effects of bvPLA2 treatment on the apoptotic signaling pathway in Treg populations. Flow cytometry was performed to identify early apoptotic cells. As a result, early apoptotic cells were dramatically decreased in bvPLA2-treated splenocytes, whereas rapamycin-treated cells showed levels of apoptotic cells similar to those of PBS-treated cells. Furthermore, bvPLA2 treatment increased expression of anti-apoptotic molecules including CTLA-4 and PD-1. The survival rate increased in bvPLA2-treated Tregs. Our findings indicate that bvPLA2-mediated modulation of apoptotic signaling is strongly associated with the Treg induction, which exhibits protective effects against various immune-related diseases. To our knowledge, this study is the first to demonstrate that bvPLA2 is the major bee venom (BV) compound capable of inducing Treg expansion through altering apoptotic signal. © 2020 by the authors.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/41605)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.