Draft genome sequence of oligosaccharide producing Leuconostoc lactis CCK940 isolated from kimchi in Korea

Abstract

Leuconostoc lactis CCK940, which was isolated from kimchi obtained from a Korean traditional market, produced an oligosaccharide with a degree of polymerization of more than 4. In this study, the draft genome sequence of L. lactis CCK940 was reported by using PacBio 20 kb platform. The genome of this strain was sequenced and the genome assembly revealed 2 contigs. The genome was 1,741,511 base pairs in size with a G + C content of 43.33%, containing 1,698 coding sequences, 12 rRNA genes, and 68 tRNA genes. L. lactis CCK940 contained genes encoding glycosyltransferase, sucrose phosphorylase, maltose phosphorylase, and β-galactosidase which could synthesize oligosaccharide. © 2018, The Microbiological Society of Korea.한국의 전통시장에서 구입한 김치에서 분리된 Leuconostoc lactis CCK940은 sucrose와 maltose를 이용하여 중합도가 4 이상인 올리고당을 생산하였다. L. lactis CCK940의 유전체는 1,741,511 bp의 2개 contig로 구성된 염색체로 조합되었으며 G + C의 비율은 43.33%로 나타났다. 염색체 DNA에서 1,698개 의 코딩 유전자, 12개의 rRNA, 68개의 tRNA 유전자가 확인되 었다. L. lactis CCK940은 올리고당을 생산할 수 있는 sucrose phosphorylase, maltose phosphorylase, β-galactosidase 등의 glucosyltransferase 생합성 유전자들을 지니고 있었다.