Impedance Characterization of Chitosan Cytotoxicity to MCF-7 Breast Cancer Cells Using a Multidisc Indium Tin Oxide Microelectrode Array
- Authors
- Park, Ilhwan; Nguyen, Tra; Park, Jinsoo; Yoo, Ah Young; Park, Jae Kweon; Cho, Sungbo
- Issue Date
- 2018
- Publisher
- ELECTROCHEMICAL SOC INC
- Citation
- JOURNAL OF THE ELECTROCHEMICAL SOCIETY, v.165, no.2, pp.B55 - B59
- Journal Title
- JOURNAL OF THE ELECTROCHEMICAL SOCIETY
- Volume
- 165
- Number
- 2
- Start Page
- B55
- End Page
- B59
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5238
- DOI
- 10.1149/2.1201802jes
- ISSN
- 0013-4651
- Abstract
- Chitosan is an amino polysaccharide with possible biomedical applications, for example, in bandage materials or as an antibacterial agent or a cytotoxic agent for tumor and cancer cells. In this study, the cytotoxic effect of chitosan (CTSN-P) on MCF-7 breast cancer cells was monitored in a nondestructive and real-time manner by electrical cell-substrate impedance sensing with a fabricated multidisc indium tin oxide electrode array. The electrical impedance characteristics of cell growth on the multidisc electrode were analyzed by equivalent electric circuit modeling. Application of CTSN-P caused deterioration of viability of cells on the electrode substrate and yielded a CTSN-P concentration-dependent decrease in impedance. The 50% cytotoxicity concentrations in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and impedimetric assay were 1266.60 and 543.17 mu g/mL, respectively. Thus, impedance measurement is suitable for detecting low-dose effects of CTSN-P on the behavior or morphology of live cells. (C) 2018 The Electrochemical Society.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - IT융합대학 > 전자공학과 > 1. Journal Articles
- 바이오나노대학 > 생명과학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.