Age-Related Changes in Nuclear Factor Erythroid 2-Related Factor 2 and Reactive Oxygen Species and Mitochondrial Structure in the Tongues of Fischer 344 Rats
- Authors
- Baek, Min-Kwan; Lee, Hyon; Kim, Kyung-Ok; Kwon, Hyun-Jin; Chung, Myung-Hee; Park, Hyoung-Min; Woo, Joo-Hyun; Kim, Dong-Young
- Issue Date
- Dec-2017
- Publisher
- KOREAN SOC OTORHINOLARYNGOL
- Keywords
- Tongue; Aging; NF-E2-Related Factor 2; Reactive Oxygen Species; Mitochondria
- Citation
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY, v.10, no.4, pp.357 - 362
- Journal Title
- CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY
- Volume
- 10
- Number
- 4
- Start Page
- 357
- End Page
- 362
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5420
- DOI
- 10.21053/ceo.2016.01095
- ISSN
- 1976-8710
- Abstract
- Objectives. Previously the authors reported age-related changes in the activities of anti-oxidative enzyme activities and protein expressions in the tongues of rats. Because more information is required about relations between aging and oxidative stress and anti-oxidative enzyme efficiency, the authors investigated differences between the expression of master regulator of anti-oxidative enzymes (nuclear factor erythroid 2-related factor 2 [Nrf2]), levels of reactive oxygen species (ROS), and mitochondrial structures in the tongues of young and aged Fischer 344 rats. Methods. Age-dependent changes in Nrf2 protein and ROS were determined by Western blotting and using chemical kits, respectively. Tongue specimens were examined by electron microscopy. The study was conducted using rats aged 7 months (young, n=8) or 22 months (old, n=8). Results. Nrf2 protein levels in the tongues of aged rats were lower than in young rats. ROS levels were higher in older rats and mitochondrial structural deficits were observed their tongues. Three young rats showed moderate mitochondrial degeneration, whereas profound degeneration with mitochondrial cristae disruption, swelling, rupture, or intramitochondrial vacuole formation was observed in all 8 old rats. Notably, mitochondrial rupture was observed in 5 old rats. Conclusion. Antioxidant defense systems of old rats were compromised by Nrf2 deficiency, which could lead to the deleterious accumulation and release of ROS and probably mitochondrial structural deficits in aged tongue tissues.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5420)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.