Bortezomib Facilitates Reparative Dentin Formation after Pulp Access Cavity Preparation in Mouse Molar
- Authors
- Jung, Jae-Kwang; Gwon, Gi-Jeong; Neupane, Sanjiv; Sohn, Wern-Joo; Kim, Ki-Rim; Kim, Ji-Youn; An, Seo-Young; Kwon, Tae-Yub; An, Chang-Hyeon; Lee, Youngkyun; Kim, Jae-Young; Ha, Jung-Hong
- Issue Date
- Dec-2017
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Cavity preparation; dentin-pulp complex; reparative dentin formation; vascularization; Wnt signaling
- Citation
- JOURNAL OF ENDODONTICS, v.43, no.12, pp.2041 - 2047
- Journal Title
- JOURNAL OF ENDODONTICS
- Volume
- 43
- Number
- 12
- Start Page
- 2041
- End Page
- 2047
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5451
- DOI
- 10.1016/j.joen.2017.07.018
- ISSN
- 0099-2399
- Abstract
- Introduction: The aim of this study was to evaluate in vitro and ex vivo roles of bortezomib, a proteasome inhibitor that binds to the active site of the 26S proteasome, in tertiary dentin formation. Methods: We established pulpal access cavity preparation that was treated with or without bortezomib before direct pulp capping with a calcium hydroxide-based material. We also analyzed bone morphogenetic protein (Bmp)and Wnt-related signaling molecules using quantitative real-time polymerase chain reaction. Results: In the short-term observation period, the bortezomib-treated pulp specimens showed the period-altered immunolocalization patterns of nestin, CD31, and myeloperoxidase, whereas the control specimens did not. The bortezomib-treated group showed a complete dentin bridge with very few irregular tubules after 42 days. The micro-computed tomographic images showed more apparent dentin bridge structures in the treated specimens than were in the controls. Quantitative real-time polymerase chain reaction analysis showed up-regulated Bmp and Wnt. Conclusions: These findings revealed that treatment with 1 mu mol/L bortezomib induced reparative dentin formation that facilitated the maintenance of the integrity of the remaining pulpal tissue via early vascularization and regulation of Bmp and Wnt signaling.
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