Protective effects of Bifidobacterium bifidum culture supernatants and intracellular cell-free extracts on human dermal fibroblasts against UV-B irradiation
- Authors
- Gwon, G.Y.; Park, G.G.
- Issue Date
- 2017
- Publisher
- Korean Society of Food Science and Nutrition
- Keywords
- Bifidobacterium bifidum; Culture supernatants; Human dermal fibroblasts; Intracellular cell free extracts
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.46, no.7, pp.801 - 808
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 46
- Number
- 7
- Start Page
- 801
- End Page
- 808
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6625
- DOI
- 10.3746/jkfn.2017.46.7.801
- ISSN
- 1226-3311
- Abstract
- The present study investigated the protective effects of Bifidobacterium bifidum culture supernatants (BbSC) and intracellular cell-free extracts (BbICFE) on human dermal fibroblasts (HDFs) against ultraviolet-B (UV-B) irradiation. HDFs were treated with UV-B, UV-B+BbCS, and UV-B+BbICFE. Treatment of UV-B-irradiated HDFs with BbCS and BbICFE significantly increased cell viability compared to UV-B-irradiated HDFs. BbCS treatment reduced senescence in HDFs by approximately 40.0%. Moreover, sub-G1 phase was significantly reduced in BbCS-and BbICFE-treated HDFs (3.3% and 4.5%, respectively). The effect of UV-B on oxidative damage of HDFs was measured by dichlorofluorescin diacetate. Fluorescence intensity significantly increased in UV-B-irradiated HDFs. Inhibition of cellular reactive oxygen species in HDFs treated with 0.01% BbCS was the highest at 34.1%. Levels of p21 and p53 protein expression induced by UV-B irradiation were reduced by treatment with BbCS and BbICFE (47.0% and 35.6%, respectively). These results show that BbCS and BbICFE reduce UV-B-induced cellular senescence and apoptosis in HDFs. Thus, BbCS and BbICFE can be used as potential agents for protection of UV-B-induced skin cell damage. © 2017, Korean Society of Food Science and Nutrition. All rights reserved.
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