Anti-adipogenic effect of neferine in 3t3-l1 cells and primary white adipocytes
DC Field | Value | Language |
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dc.contributor.author | Park M. | - |
dc.contributor.author | Han J. | - |
dc.contributor.author | Lee H.-J. | - |
dc.date.available | 2020-07-30T00:35:53Z | - |
dc.date.created | 2020-07-03 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/71659 | - |
dc.description.abstract | Neferine, an alkaloid component extracted from lotus seed embryos, is known for its anti-inflammatory, anticancer, and antioxidant properties. However, the anti-adipogenic activity of neferine has not been thoroughly investigated. In this study, neferine was found to inhibit lipid accumulation in a dose-dependent manner during the differentiation of 3T3-L1 cells without inducing cytotoxicity. Real-time polymerase chain reaction and immunoblot analysis revealed the downregulation in the expression of peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element-binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) and the upregulation in carnitine palmitoyltransferase-1 (CPT-1) and sirtuin 1 (SIRT1) levels following neferine treatment. Furthermore, neferine increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), which is an important regulator of fatty acid oxidation. Our result indicates that neferine attenuates adipogenesis and promotes lipid metabolism by activating AMPK-mediated signaling. Therefore, neferine may serve as a therapeutic candidate for obesity treatment. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.relation.isPartOf | Nutrients | - |
dc.title | Anti-adipogenic effect of neferine in 3t3-l1 cells and primary white adipocytes | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000549197500001 | - |
dc.identifier.doi | 10.3390/nu12061858 | - |
dc.identifier.bibliographicCitation | Nutrients, v.12, no.6, pp.1 - 12 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85086937693 | - |
dc.citation.endPage | 12 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Nutrients | - |
dc.citation.volume | 12 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Park M. | - |
dc.contributor.affiliatedAuthor | Han J. | - |
dc.contributor.affiliatedAuthor | Lee H.-J. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 3T3-L1 preadipocytes | - |
dc.subject.keywordAuthor | Anti-adipogenic activity | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Neferine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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