Anti-adipogenic effect of neferine in 3t3-l1 cells and primary white adipocytes
- Authors
- Park M.; Han J.; Lee H.-J.
- Issue Date
- Jun-2020
- Publisher
- MDPI AG
- Keywords
- 3T3-L1 preadipocytes; Anti-adipogenic activity; Differentiation; Neferine
- Citation
- Nutrients, v.12, no.6, pp.1 - 12
- Journal Title
- Nutrients
- Volume
- 12
- Number
- 6
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/71659
- DOI
- 10.3390/nu12061858
- ISSN
- 2072-6643
- Abstract
- Neferine, an alkaloid component extracted from lotus seed embryos, is known for its anti-inflammatory, anticancer, and antioxidant properties. However, the anti-adipogenic activity of neferine has not been thoroughly investigated. In this study, neferine was found to inhibit lipid accumulation in a dose-dependent manner during the differentiation of 3T3-L1 cells without inducing cytotoxicity. Real-time polymerase chain reaction and immunoblot analysis revealed the downregulation in the expression of peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), sterol regulatory element-binding protein-1c (SREBP-1c), and fatty acid synthase (FAS) and the upregulation in carnitine palmitoyltransferase-1 (CPT-1) and sirtuin 1 (SIRT1) levels following neferine treatment. Furthermore, neferine increased the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), which is an important regulator of fatty acid oxidation. Our result indicates that neferine attenuates adipogenesis and promotes lipid metabolism by activating AMPK-mediated signaling. Therefore, neferine may serve as a therapeutic candidate for obesity treatment. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 식품영양학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.