Antioxidant Activity of Chitosan Hydrolysates Derived by Microwave-assisted Irradiation

Abstract

Degradation of chitosan by free radical cleavage at 90 oC in the presence of ammonium persulfate was performed. Typically, high molecular weight chitosan was dissolved in 2% acetic acid and then submitted to the Microwave-assisted and room temperature using different time. The in vitro quantification analysis and antioxidant activity of self-assembled and hydrolyzed chitosan was evaluated and compared as radical scavengers against 1,1- diphenyl-2-picrylhydrazyl radicals (DPPH•), 2,2’- Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) using established methods and the effect of microwave and room temperature on the antioxidant activity of these molecular weight chitosan is discussed. Our data suggested the different type chitosan hydrolysates could be effectively employed as natural antioxidant materials for the various applications such as cosmeceutical and other potent industry products.