Synergistic Effects of Bee Venom and Natural Killer Cells on B16F10 Melanoma Cell Growth Inhibition through IL-4-mediated Apoptosis
DC Field | Value | Language |
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dc.contributor.author | 신대철 | - |
dc.contributor.author | 강미숙 | - |
dc.contributor.author | 송호섭 | - |
dc.date.available | 2020-02-27T22:43:51Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2586-288X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7408 | - |
dc.description.abstract | Objectives : We investigated the synergistic effects of bee venom (BV) and natural killer (NK) cells on B16F10 melanoma cell apoptosis mediated by IL-4. Methods : We performed a cell viability assay to determine whether BV can enhance the inhibitory effect of NK-92MI cells on the growth of B16F10 melanoma cells, and western blot analysis to detect changes in the expression of IL-4, IL-4Rα, and other apoptosis-related proteins. EMSA was performed to observe the activity of STAT6. To confirm that the inhibitory effect of BV and NK cells was mediated by IL-4, the above tests were repeated after IL-4 silencing by siRNA (50 nM). Results : B16F10 melanoma cells co-cultured with NK-92MI cells and simultaneously treated by BV (5 μg/ml) showed a higher degree of proliferation inhibition than when treated by BV (5 μg/ml) alone or co-cultured with NK-92MI cells alone. Expression of IL-4, IL-4Rα, and that of other pro-apoptotic proteins was also enhanced after co-culture with NK-92MI cells and simultaneous treatment with BV (5 μg/ml). Furthermore, the expression of anti-apoptotic bcl- 2 decreased, and the activity of STAT6, as well as the expression of STAT6 and p-STAT6 were enhanced. IL-4 silencing siRNA (50 nM) in B16F10 cells, the effects of BV treatment and NK-92MI co-culture were reversed. Conclusion : These results suggest that BV could be an effective alternative therapy for malignant melanoma by enhancing the cytotoxic and apoptotic effect of NK cells through an IL-4-mediated pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한침구의학회 | - |
dc.relation.isPartOf | Journal of Acupuncture Research | - |
dc.title | Synergistic Effects of Bee Venom and Natural Killer Cells on B16F10 Melanoma Cell Growth Inhibition through IL-4-mediated Apoptosis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | Journal of Acupuncture Research, v.34, no.1, pp.1 - 9 | - |
dc.identifier.kciid | ART002196326 | - |
dc.citation.endPage | 9 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Journal of Acupuncture Research | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 신대철 | - |
dc.contributor.affiliatedAuthor | 강미숙 | - |
dc.contributor.affiliatedAuthor | 송호섭 | - |
dc.subject.keywordAuthor | Bee Venom | - |
dc.subject.keywordAuthor | Malignant Melanoma | - |
dc.subject.keywordAuthor | IL-4 | - |
dc.subject.keywordAuthor | Natural Killer cell | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.description.journalRegisteredClass | kci | - |
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