Synergistic Effects of Bee Venom and Natural Killer Cells on B16F10 Melanoma Cell Growth Inhibition through IL-4-mediated Apoptosis
- Authors
- 신대철; 강미숙; 송호섭
- Issue Date
- 2017
- Publisher
- 대한침구의학회
- Keywords
- Bee Venom; Malignant Melanoma; IL-4; Natural Killer cell; Apoptosis
- Citation
- Journal of Acupuncture Research, v.34, no.1, pp.1 - 9
- Journal Title
- Journal of Acupuncture Research
- Volume
- 34
- Number
- 1
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7408
- ISSN
- 2586-288X
- Abstract
- Objectives : We investigated the synergistic effects of bee venom (BV) and natural killer (NK) cells on B16F10 melanoma cell apoptosis mediated by IL-4.
Methods : We performed a cell viability assay to determine whether BV can enhance the inhibitory effect of NK-92MI cells on the growth of B16F10 melanoma cells, and western blot analysis to detect changes in the expression of IL-4, IL-4Rα, and other apoptosis-related proteins. EMSA was performed to observe the activity of STAT6. To confirm that the inhibitory effect of BV and NK cells was mediated by IL-4, the above tests were repeated after IL-4 silencing by siRNA (50 nM).
Results : B16F10 melanoma cells co-cultured with NK-92MI cells and simultaneously treated by BV (5 μg/ml) showed a higher degree of proliferation inhibition than when treated by BV (5 μg/ml) alone or co-cultured with NK-92MI cells alone. Expression of IL-4, IL-4Rα, and that of other pro-apoptotic proteins was also enhanced after co-culture with NK-92MI cells and simultaneous treatment with BV (5 μg/ml). Furthermore, the expression of anti-apoptotic bcl- 2 decreased, and the activity of STAT6, as well as the expression of STAT6 and p-STAT6 were enhanced. IL-4 silencing siRNA (50 nM) in B16F10 cells, the effects of BV treatment and NK-92MI co-culture were reversed.
Conclusion : These results suggest that BV could be an effective alternative therapy for malignant melanoma by enhancing the cytotoxic and apoptotic effect of NK cells through an IL-4-mediated pathway.
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