Superior antibacterial activity of GlcN-AuNP-GO by ultraviolet irradiation

Abstract

A complete bacterialysis analysis of glucosamine-gold nanoparticle-graphene oxide (GIcN-AuNP-GO) and UV-irradiated GIcN-AuNP-GO was conducted. Analytical characterization of GlcN-AuNPs, GO and GIcN-AuNP-GO revealed UV-Vis absorbance peak at around 230 and 500 nm. Microscopic characterization of prepared nanomaterials was performed by scanning electron microscope, atomic force microscopy, and high-resolution transmission microscopy. The results confirmed that the GIcN-AuNPs were uniformly decorated on the surface and edges of graphene sheets. In addition, potent antibacterial activity of GIcN-AuNP-GO that was UV irradiated for 10 min and normal GIcN-AuNP-GO was detected, compared to the standard drug kanamycin, against both Gram-negative and positive bacteria. The minimum inhibitory concentration (MIC) and fluorescence intensity spectra results for Escherichia colt and Enterococcus faecalis showed that the UV-irradiated GIcN-AuNP-GO has better antibacterial activity than normal GIcN-AuNP-GO and kanamycin. Morphological changes were detected by AFM after treatment. These results confirmed that GIcN-AuNP-GO is a potent antibacterial agent with good potential for use in manufacturing medical instruments, pharmaceutical industries and in waste water treatment (C) 2016 Published by Elsevier B.V.