Expression of thaumatin, a new type of alternative sweetener in rice
- Authors
- Akter, S.; Huq, M.A.; Jung, Y.-J.; Kang, K.-K.
- Issue Date
- Jul-2020
- Publisher
- Academic Press
- Keywords
- Agrobacterium tumefaciens; Oryza sativa L; Protein expression; Taqman PCR; Thaumatin
- Citation
- Notulae Botanicae Horti Agrobotanici Cluj-Napoca, v.48, no.3, pp.1276 - 1291
- Journal Title
- Notulae Botanicae Horti Agrobotanici Cluj-Napoca
- Volume
- 48
- Number
- 3
- Start Page
- 1276
- End Page
- 1291
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78854
- DOI
- 10.15835/nbha48311676
- ISSN
- 0255-965X
- Abstract
- Sweet proteins are the natural alternative to the artificial sweeteners as well as flavor enhancers. Among other sweet protein, thaumatin protein was isolated from Thaumatococcus daniellii Benth plant fruit. In this study, pinII Ti plasmid vector was constructed with thaumatin gene, where thaumatin was placed under the control of the duel cauliflower mosaic virus 35S promoter into rice (Oryza sativa L. var. japonica cv. 'Dongjinbyeo') by Agrobacterium-mediated transformation to generate transgenic plants. Thirteen plant lines were regenerated and the transgenic rice lines were confirmed by different molecular analysis. The genomic PCR result revealed that all of the plant lines were transgenic. The single copy and intergenic plant lines were selected by Taqman PCR analysis and FST analysis, respectively. Expression of thaumatin gene in transgenic rice resulted in the accumulation of thaumatin protein in the leave. Thaumatin protein was also accumulated in leave of T1 generation. Sensory analysis result suggested that the thaumatin protein expressing transgenic lines exerted sweet tasting activity. These results demonstrated that thaumatin was expressed in transgenic rice plants. © Articles by the authors; UASVM.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.