Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7
DC Field | Value | Language |
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dc.contributor.author | 박도원 | - |
dc.contributor.author | 박종현 | - |
dc.date.available | 2020-11-12T00:40:03Z | - |
dc.date.created | 2020-11-03 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78924 | - |
dc.description.abstract | With an increase in the consumption of non-heated fresh food, foodborne shiga toxin-producing Escherichia coli (STEC) has emerged as one of the most problematic pathogens worldwide. Endolysin, a bacteriophage-derived lysis protein, is able to lyse the target bacteria without any special resistance, and thus has been garnering interest as a powerful antimicrobial agent. In this study, rV5-like phage endolysin targeting E. coli O157:H7, named as LysECP26, was identified and purified. This endolysin had a lysozyme-like catalytic domain, but differed markedly from the sequence of lambda phage endolysin. LysECP26 exhibited strong activity with a broad lytic spectrum against various gram-negative strains (29/29) and was relatively stable at a broad temperature range (4°C– 55°C). The optimum temperature and pH ranges of LysECP26 were identified at 37°C–42°C and pH 7– 8, respectively. NaCl supplementation did not affect the lytic activity. Although LysECP26 was limited in that it could not pass the outer membrane, E. coli O157: H7 could be effectively controlled by adding ethylenediaminetetraacetic acid (EDTA) and citric acid (1.44 and 1.14 log CFU/ml) within 30 min. Therefore, LysECP26 may serve as an effective biocontrol agent for gram-negative pathogens, including E. coli O157:H7. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.relation.isPartOf | Journal of Microbiology and Biotechnology | - |
dc.title | Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7 | - |
dc.title.alternative | Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000582806600013 | - |
dc.identifier.doi | 10.4014/jmb.2005.05030 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.30, no.10, pp.1552 - 1558 | - |
dc.identifier.kciid | ART002640305 | - |
dc.identifier.scopusid | 2-s2.0-85094933215 | - |
dc.citation.endPage | 1558 | - |
dc.citation.startPage | 1552 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 30 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | 박도원 | - |
dc.contributor.affiliatedAuthor | 박종현 | - |
dc.subject.keywordAuthor | E. coli O157:H7 | - |
dc.subject.keywordAuthor | rV5-like phage | - |
dc.subject.keywordAuthor | endolysin | - |
dc.subject.keywordAuthor | outer membrane permeabilizers (OMPs) | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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