Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7
- Other Titles
- Characterization of Endolysin LysECP26 Derived from rV5-like Phage vB_EcoM-ECP26 for Inactivation of Escherichia coli O157:H7
- Authors
- 박도원; 박종현
- Issue Date
- Oct-2020
- Publisher
- 한국미생물·생명공학회
- Keywords
- E. coli O157:H7; rV5-like phage; endolysin; outer membrane permeabilizers (OMPs)
- Citation
- Journal of Microbiology and Biotechnology, v.30, no.10, pp.1552 - 1558
- Journal Title
- Journal of Microbiology and Biotechnology
- Volume
- 30
- Number
- 10
- Start Page
- 1552
- End Page
- 1558
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78924
- DOI
- 10.4014/jmb.2005.05030
- ISSN
- 1017-7825
- Abstract
- With an increase in the consumption of non-heated fresh food, foodborne shiga toxin-producing Escherichia coli (STEC) has emerged as one of the most problematic pathogens worldwide. Endolysin, a bacteriophage-derived lysis protein, is able to lyse the target bacteria without any special resistance, and thus has been garnering interest as a powerful antimicrobial agent. In this study, rV5-like phage endolysin targeting E. coli O157:H7, named as LysECP26, was identified and purified.
This endolysin had a lysozyme-like catalytic domain, but differed markedly from the sequence of lambda phage endolysin. LysECP26 exhibited strong activity with a broad lytic spectrum against various gram-negative strains (29/29) and was relatively stable at a broad temperature range (4°C– 55°C). The optimum temperature and pH ranges of LysECP26 were identified at 37°C–42°C and pH 7– 8, respectively. NaCl supplementation did not affect the lytic activity. Although LysECP26 was limited in that it could not pass the outer membrane, E. coli O157: H7 could be effectively controlled by adding ethylenediaminetetraacetic acid (EDTA) and citric acid (1.44 and 1.14 log CFU/ml) within 30 min. Therefore, LysECP26 may serve as an effective biocontrol agent for gram-negative pathogens, including E. coli O157:H7.
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