Flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with SYBR green i and CD235A
DC Field | Value | Language |
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dc.contributor.author | Jang, J.W. | - |
dc.contributor.author | Kim, J.Y. | - |
dc.contributor.author | Yoon, J. | - |
dc.contributor.author | Yoon, S.Y. | - |
dc.contributor.author | Cho, C.H. | - |
dc.contributor.author | Han, E.T. | - |
dc.contributor.author | An, S.S.A. | - |
dc.contributor.author | Lim, C.S. | - |
dc.date.available | 2020-12-15T08:40:23Z | - |
dc.date.created | 2020-12-15 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 2356-6140 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79339 | - |
dc.description.abstract | A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2-37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2-66.0%). High correlation of measured parasitaemia (r = 0.98, P < 0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients. © 2014 Jin Woo Jang et al. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Hindawi Limited | - |
dc.relation.isPartOf | Scientific World Journal | - |
dc.title | Flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with SYBR green i and CD235A | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.1155/2014/536723 | - |
dc.identifier.bibliographicCitation | Scientific World Journal, v.2014 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84919723244 | - |
dc.citation.title | Scientific World Journal | - |
dc.citation.volume | 2014 | - |
dc.contributor.affiliatedAuthor | An, S.S.A. | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CD235A PE | - |
dc.subject.keywordPlus | fluorescent dye | - |
dc.subject.keywordPlus | propidium iodide | - |
dc.subject.keywordPlus | sybr green I | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | Giemsa stain | - |
dc.subject.keywordPlus | glycophorin | - |
dc.subject.keywordPlus | organic compound | - |
dc.subject.keywordPlus | SYBR Green I | - |
dc.subject.keywordPlus | accuracy | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | blood smear | - |
dc.subject.keywordPlus | culture medium | - |
dc.subject.keywordPlus | culture technique | - |
dc.subject.keywordPlus | erythrocyte | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | fluorescence activated cell sorting | - |
dc.subject.keywordPlus | fluorescence microscope | - |
dc.subject.keywordPlus | Giemsa stain | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | intermethod comparison | - |
dc.subject.keywordPlus | microscopy | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | parasitemia | - |
dc.subject.keywordPlus | Plasmodium falciparum | - |
dc.subject.keywordPlus | sensitivity and specificity | - |
dc.subject.keywordPlus | stain | - |
dc.subject.keywordPlus | staining | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | Malaria, Falciparum | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | parasitemia | - |
dc.subject.keywordPlus | parasitology | - |
dc.subject.keywordPlus | physiology | - |
dc.subject.keywordPlus | Plasmodium falciparum | - |
dc.subject.keywordPlus | procedures | - |
dc.subject.keywordPlus | staining | - |
dc.subject.keywordPlus | Plasmodium falciparum | - |
dc.subject.keywordPlus | Azure Stains | - |
dc.subject.keywordPlus | Erythrocytes | - |
dc.subject.keywordPlus | Flow Cytometry | - |
dc.subject.keywordPlus | Glycophorin | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Malaria, Falciparum | - |
dc.subject.keywordPlus | Microscopy | - |
dc.subject.keywordPlus | Organic Chemicals | - |
dc.subject.keywordPlus | Parasitemia | - |
dc.subject.keywordPlus | Plasmodium falciparum | - |
dc.subject.keywordPlus | Staining and Labeling | - |
dc.description.journalRegisteredClass | scopus | - |
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