Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte
DC Field | Value | Language |
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dc.contributor.author | Hwang, S. | - |
dc.contributor.author | Shin, D.M. | - |
dc.contributor.author | Hong, J.H. | - |
dc.date.available | 2020-12-16T01:40:44Z | - |
dc.date.created | 2020-11-20 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79355 | - |
dc.description.abstract | Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
dc.title | Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000594945600001 | - |
dc.identifier.doi | 10.3390/ijms21228429 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.21, no.22, pp.1 - 17 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85096026062 | - |
dc.citation.endPage | 17 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 21 | - |
dc.citation.number | 22 | - |
dc.contributor.affiliatedAuthor | Hwang, S. | - |
dc.contributor.affiliatedAuthor | Hong, J.H. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Anion exchanger 2 | - |
dc.subject.keywordAuthor | Histamine | - |
dc.subject.keywordAuthor | Intracellular Ca2+ | - |
dc.subject.keywordAuthor | Keratinocyte migration | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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