Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte
- Authors
- Hwang, S.; Shin, D.M.; Hong, J.H.
- Issue Date
- Nov-2020
- Publisher
- MDPI AG
- Keywords
- Anion exchanger 2; Histamine; Intracellular Ca2+; Keratinocyte migration
- Citation
- International Journal of Molecular Sciences, v.21, no.22, pp.1 - 17
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 21
- Number
- 22
- Start Page
- 1
- End Page
- 17
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79355
- DOI
- 10.3390/ijms21228429
- ISSN
- 1661-6596
- Abstract
- Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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