The Flavonol Isoquercitrin Promotes Mitochondrial-Dependent Apoptosis in SK-Mel-2 Melanoma Cell via the PI3K/AKT/mTOR Pathway
DC Field | Value | Language |
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dc.contributor.author | Won, Y.-S. | - |
dc.contributor.author | Kim, J.-H. | - |
dc.contributor.author | Lizardo, R.C.M. | - |
dc.contributor.author | Min, H.-J. | - |
dc.contributor.author | Cho, H.-D. | - |
dc.contributor.author | Hong, S.-M. | - |
dc.contributor.author | Seo, K.-I. | - |
dc.date.available | 2021-01-11T00:40:33Z | - |
dc.date.created | 2020-12-09 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79677 | - |
dc.description.abstract | Isoquercitrin (IQ), a major flavonol present in Prunus mume fruit, has gained much attention in recent studies because of its superior bioavailability and physiological effects. In this study, the anti-cancer mechanism of IQ against human melanoma, particularly its effect on the mitochondria-mediated apoptosis, was investigated. Treatment with IQ at 25 μM concentration effectively inhibited the proliferation of SK-MEL-2 skin cancer cells while the same concentration did not exhibit cytotoxicity against human keratinocytes HaCaT. Morphological analysis and clonogenic assay also showed that IQ can alter the growth and long-term survival of SK-MEL-2 cells. IQ also induced apoptosis in the melanoma cells as manifested in the nuclear morphology changes, DNA fragmentation, increase in the apoptosis rate (17.69% at 25 μM) and accumulation of sub-G1 cell cycle phase population (19.55% at 25 μM). Western blot analysis revealed the involvement of the mitochondrial apoptosis signaling pathway in the anti-cancer property of IQ. Treatment with IQ resulted in the decrease in the levels of procaspase-8 and -9, and Bcl-2 protein, and an increase in the expression of cleaved PARP and Bax. Moreover, AIF and Endo G protein expression increased, indicating a caspase-independent mitochondrial-mediated apoptosis. The anti-proliferative activity of IQ against SK-MEL-2 can also be attributed to the downregulation of the PI3K/AktmTOR signaling pathway. These findings showed that IQ can be developed into a chemopreventive therapeutic agent against the melanoma cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | Nutrients | - |
dc.title | The Flavonol Isoquercitrin Promotes Mitochondrial-Dependent Apoptosis in SK-Mel-2 Melanoma Cell via the PI3K/AKT/mTOR Pathway | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000602545300001 | - |
dc.identifier.doi | 10.3390/nu12123683 | - |
dc.identifier.bibliographicCitation | Nutrients, v.12, no.12 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85097033952 | - |
dc.citation.title | Nutrients | - |
dc.citation.volume | 12 | - |
dc.citation.number | 12 | - |
dc.contributor.affiliatedAuthor | Hong, S.-M. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | flavonol | - |
dc.subject.keywordAuthor | human melanoma | - |
dc.subject.keywordAuthor | isoquercitrin | - |
dc.subject.keywordAuthor | mitochondrial-mediated apoptosis | - |
dc.subject.keywordAuthor | natural chemopreventive agent | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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