In Vivo and In Vitro Quantification of Glucose Kinetics: From Bedside to Bench
- Authors
- Kim, Il-Young; Park, Sanghee; Kim, Yeongmin; Chang, Yewon; Choi, Cheol Soo; Suh, Sang-Hoon; Robert R. Wolfe
- Issue Date
- Dec-2020
- Publisher
- 대한내분비학회
- Keywords
- Stable isotope tracers; Metabolic fluxomics; Insulin resistance; Diabetes mellitus
- Citation
- Endocrinology and Metabolism, v.35, no.4, pp.733 - 749
- Journal Title
- Endocrinology and Metabolism
- Volume
- 35
- Number
- 4
- Start Page
- 733
- End Page
- 749
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79838
- DOI
- 10.3803/EnM.2020.406
- ISSN
- 2093-596X
- Abstract
- Like other substrates, plasma glucose is in a dynamic state of constant turnover (i.e., rates of glucose appearance [Ra glucose] into and disappearance [Rd glucose] from the plasma) while staying within a narrow range of normal concentrations, a physiological priority. Persistent imbalance of glucose turnover leads to elevations (i.e., hyperglycemia, Ra>Rd) or falls (i.e., hypoglycemia, Ra<Rd) in the pool size, leading to clinical conditions such as diabetes. Endogenous Ra glucose is divided into hepatic glucose production via glycogenolysis and gluconeogenesis (GNG) and renal GNG. On the other hand, Rd glucose, the summed rate of glucose uptake by tissues/organs, involves various intracellular metabolic pathways including glycolysis, the tricarboxylic acid (TCA) cycle, and oxidation at varying rates depending on the metabolic status. Despite the dynamic nature of glucose metabolism, metabolic studies typically rely on measurements of static, snapshot information such as the abundance of mRNAs and proteins and (in)activation of implicated signaling networks without determining actual flux rates. In this review, we will discuss the importance of obtaining kinetic information, basic principles of stable isotope tracer methodology, calculations of in vivo glucose kinetics, and assessments of metabolic flux in experimental models in vivo and in vitro.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/79838)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.