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Double-Stranded DNA-Templated Copper Nanoclusters for Detection of DNA Polymerase Activity
- Authors
- Kim, Jungeun; Gang, Jongback
- Issue Date
- Jan-2021
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- DNA& #8208; templated copper nanoclusters; DNA& #8208; CuNCs; Biosensor; DNA polymerase; Klenow fragment
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.42, no.1, pp.32 - 36
- Journal Title
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY
- Volume
- 42
- Number
- 1
- Start Page
- 32
- End Page
- 36
- ISSN
- 0253-2964
- Abstract
- DNA polymerase plays an important role in the proliferation and survival of all forms of life and the variety of biotechnological applications. In this study, DNA-templated copper nanoclusters were utilized to detect Klenow fragment exo- (KF-) activity and monitor its inhibition in the presence of 5-fluorouracil by measurement of fluorescence intensities. This study showed that the fluorescence intensities increased continuously for KF- concentrations ranging from 0 to 5 U/mL. In addition, inhibition of KF- activity was measured at 5-fluorouracil concentrations ranging from 0 to 150 mu M. Using nonlinear regression, the corresponding IC50 was determined to be 6.23 mu M in the presence of 5.0 U/mL KF-.
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Related Researcher
- Gang, Jong Back
- BioNano Technology (Department of Chemistry)