Optimization and characterization of covalent immobilization of glucose oxidase for bioelectronic devices
- Authors
- Wang, Xue; Kim, Sung Sae; Khang, Dongwoo; Kim, Hyug-Han; Kim, Chang-Joon
- Issue Date
- 15-Aug-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Immobilized enzyme; Multi-walled carbon nanotubes; Optimization; Covalent immobilization; Biosensor; Biofuel cell
- Citation
- BIOCHEMICAL ENGINEERING JOURNAL, v.112, pp.20 - 31
- Journal Title
- BIOCHEMICAL ENGINEERING JOURNAL
- Volume
- 112
- Start Page
- 20
- End Page
- 31
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8009
- DOI
- 10.1016/j.bej.2016.03.016
- ISSN
- 1369-703X
- Abstract
- Enzyme electrodes are widely applied to miniature implantable bioelectronic devices such as biofuel cells and biosensors. The main obstacle associated with miniaturization is the reduced surface area of electrodes for the accommodation of enzymes, leading to poor power output or detection signals. This study aimed to maximize the loading of glucose oxidase (GOx) on the surface of multi-walled carbon nanotubes (MWCNTs), thereby enhancing the generation of electric power or sensing signals. Because the concentrations of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), and glucose oxidase significantly affected the immobilization efficiency, these factors were optimized by the Box-Behnken design. The physically adsorbed enzyme was almost completely removed by washing the GOx-bound MWCNTs with buffer solution containing 5 g/L of Tween-20. Enzyme loading was found to be similar to 3.3 +/- 0.3 mg-GOx/mg-MWCNTs under the optimal conditions (430 mM NHS, 52 mM EDC and 8.7 mg/mL GOx). The formation of carboxyl group on the surface of MWCNTs and the covalent bonding between GOx and MWCNTs, and immobilized GOx were observed by FTIR and AFM, respectively. The biochemical analysis showed that the immobilized GOx possesses high activity for the conversion of glucose into gluconic acid. The cyclic voltammetry data showed that the anodic current density of electrodes loaded with the highest amount of GOx was much higher than those of electrodes loaded with smaller amounts of GOx. (c) 2016 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8009)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.