Sphingomonas hortisp. nov., a novel bacterial species isolated from soil of a tomato garden
- Authors
- Akter, S.; Lee, S.-Y.; Moon, S.-K.; Choi, C.; Balusamy, S.R.; Siddiqi, M.Z.; Ashrafudoulla, M.; Huq, M.A.
- Issue Date
- Mar-2021
- Publisher
- SPRINGER
- Keywords
- 16S rRNA; Genome sequence; Gram-staining negative; Sphingomonas horti
- Citation
- Archives of Microbiology, v.203, no.2, pp.543 - 548
- Journal Title
- Archives of Microbiology
- Volume
- 203
- Number
- 2
- Start Page
- 543
- End Page
- 548
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80096
- DOI
- 10.1007/s00203-020-02056-x
- ISSN
- 0302-8933
- Abstract
- A novel bacterial strain, designated MAH-20T, was isolated from a soil sample of a tomato garden. Cells of strain MAH-20T were Gram-stain negative, aerobic, motile, and rod-shaped. The colonies were light brown colored, smooth, spherical, and 0.2–0.7 mm in diameter when grown on Luria–Bertani agar for 2 days. Strain MAH-20T grows at 15–40 °C (optimum growth temperature 30–32 °C), at pH 5.0–10.0 (optimum growth pH 7.0) and at 0–2.0% NaCl. The strain showed positive activity for both oxidase and catalase tests. Cells were able to hydrolyze starch, DNA, urea, gelatin, L-arginine, and Tween 20. According to the 16S rRNA gene sequence similarity, the strain MAH-20T was identified as a new member of the genus Sphingomonas and had the close sequence similarity with Sphingomonas changbaiensis V2M44T (98.9%) and Sphingomonas tabacisoli X1-8T (98.1%). The genomic ANI value between strain MAH-20T and S. changbaiensis NBRC 104936T was 84.4%. The novel strain MAH-20T has a draft genome size of 3,350,026 bp (25 contigs), annotated with 3210 protein-coding genes, 46 tRNA, and 3 rRNA genes. The genomic DNA G + C content of isolate was 67.3 mol%, the predominant quinone was ubiquinone 10 and the major fatty acids were C16:0, C17:1ω6c and summed feature 8 (comprising C18:1ω7c and/or C18:1ω6c). On the basis of DNA-DNA hybridization results, phenotypic, genotypic, and chemotaxonomic data, the isolated strain MAH-20T represents a novel species, for which the name Sphingomonas horti sp. nov. is proposed, with MAH-20T as the type strain (= KACC 19746T = CGMCC1.13658T). © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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