Identification of anti-HIV and anti-Reverse Transcriptase activity from Tetracera scandens
- Authors
- Kwon, Hyeok Sang; Park, Jung Ae; Kim, Joo-Hwan; You, Ji Chang
- Issue Date
- Mar-2012
- Publisher
- 생화학분자생물학회
- Keywords
- Anti-HIV agent(s); Anti-HIV Reverse Transcriptase; HIV-1; Tetracera scandens
- Citation
- BMB Reports, v.45, no.3, pp.165 - 170
- Journal Title
- BMB Reports
- Volume
- 45
- Number
- 3
- Start Page
- 165
- End Page
- 170
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/80791
- DOI
- 10.5483/BMBRep.2012.45.3.165
- ISSN
- 1976-6696
- Abstract
- We report here that an ethanol extract of Tetracera scandens, a Vietnamese medicinal plant, has anti-HIV activity and possesses strong inhibitory activity against HIV-1 reverse transcriptase (RTase). Using a MT-4 cell-based assay, we found that the T. scandens extract inhibited effectively HIV virus replication with an IC50 value in the range of 2.0-2.5 μg/ml while the cellular toxicity value (CC50) was more than 40-50 μg/ml concentration, thus yielding a minimum specificity index of 20-fold. Moreover, the anti-HIV efficacy of the T. scandens extract was determined to be due, in part, to its potent inhibitory activity against HIV-1 RTase activity in vitro. The inhibitory activity against the RTase was further confirmed by probing viral cDNA production, an intermediate of viral reverse transcription,in virus-infected cells using quantitative DNA-PCR analysis. Thus, these results suggest that T. scandens can be a useful source for the isolation and development of new anti-HIV-1 inhibitor(s). [BMB reports 2012; 45(3): 165-170]
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