Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning
DC Field | Value | Language |
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dc.contributor.author | Park, Do-won | - |
dc.contributor.author | Lee, Young-Duck | - |
dc.contributor.author | Park, Jong-Hyun | - |
dc.date.accessioned | 2021-06-02T01:40:24Z | - |
dc.date.available | 2021-06-02T01:40:24Z | - |
dc.date.created | 2021-05-24 | - |
dc.date.issued | 2021-05 | - |
dc.identifier.issn | 1226-7708 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81183 | - |
dc.description.abstract | To reduce staphylococcal food poisoning (SFP), a phage-encoded cell wall hydrolase called endolysin has emerged as an attractive antibacterial agent. In this study, the Staphylococcus aureus infecting phage vB_SauS-SAP27 (phi SAP27) was isolated from sewage and characterized morphologically and genetically. phi SAP27 was identified as Siphoviridae temperate phage, with a genome of 43 kbp. A phi SAP27 endolysin named LysSAP27 was produced recombinantly in Escherichia coli. LysSAP27 exhibited the highest activity at neutral pH and a temperature of 30 degrees C, and its lytic activity was upregulated by calcium ions. Following optimization of the enzymatic conditions, LysSAP27 was applied to S. aureus-contaminated milk. Treatment with 2 mu M LysSAP27 led to a significant bactericidal effect, corresponding to a reduction in bacterial titer by 2.8 log CFU/mL within 1 h and 3.4 log CFU/mL within 2 h. Therefore, LysSAP27 could be used as an effective antimicrobial agent to prevent SFP in food. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST | - |
dc.relation.isPartOf | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.title | Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000649363500001 | - |
dc.identifier.doi | 10.1007/s10068-021-00910-2 | - |
dc.identifier.bibliographicCitation | FOOD SCIENCE AND BIOTECHNOLOGY, v.30, no.5, pp.745 - 753 | - |
dc.identifier.kciid | ART002717640 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85105960851 | - |
dc.citation.endPage | 753 | - |
dc.citation.startPage | 745 | - |
dc.citation.title | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Park, Do-won | - |
dc.contributor.affiliatedAuthor | Park, Jong-Hyun | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | Endolysin | - |
dc.subject.keywordAuthor | Staphylococcus aureus | - |
dc.subject.keywordAuthor | Food Poisoning | - |
dc.subject.keywordPlus | BIOCONTROL AGENT | - |
dc.subject.keywordPlus | AUREUS | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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