Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning
- Authors
- Park, Do-won; Lee, Young-Duck; Park, Jong-Hyun
- Issue Date
- May-2021
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- Bacteriophage; Endolysin; Staphylococcus aureus; Food Poisoning
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.30, no.5, pp.745 - 753
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 30
- Number
- 5
- Start Page
- 745
- End Page
- 753
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81183
- DOI
- 10.1007/s10068-021-00910-2
- ISSN
- 1226-7708
- Abstract
- To reduce staphylococcal food poisoning (SFP), a phage-encoded cell wall hydrolase called endolysin has emerged as an attractive antibacterial agent. In this study, the Staphylococcus aureus infecting phage vB_SauS-SAP27 (phi SAP27) was isolated from sewage and characterized morphologically and genetically. phi SAP27 was identified as Siphoviridae temperate phage, with a genome of 43 kbp. A phi SAP27 endolysin named LysSAP27 was produced recombinantly in Escherichia coli. LysSAP27 exhibited the highest activity at neutral pH and a temperature of 30 degrees C, and its lytic activity was upregulated by calcium ions. Following optimization of the enzymatic conditions, LysSAP27 was applied to S. aureus-contaminated milk. Treatment with 2 mu M LysSAP27 led to a significant bactericidal effect, corresponding to a reduction in bacterial titer by 2.8 log CFU/mL within 1 h and 3.4 log CFU/mL within 2 h. Therefore, LysSAP27 could be used as an effective antimicrobial agent to prevent SFP in food.
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