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Establishment of particulate matter-induced lung injury model in mouse

Authors
Park, Se YongAn, Kyu SupLee, BuhyunKang, Ju-HeeJung, Hyun JinKim, Min WooRyu, Hyeon YeolShim, Kyu-SukNam, Ki TaekYoon, Yeo SungOh, Seung Hyun
Issue Date
30-Jul-2021
Publisher
한국실험동물학회
Keywords
Air pollution; Particulate matter; Animal model; Lung injury; Asthma
Citation
LABORATORY ANIMAL RESEARCH, v.37, no.3, pp.252 - 263
Journal Title
LABORATORY ANIMAL RESEARCH
Volume
37
Number
3
Start Page
252
End Page
263
URI
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/81862
DOI
10.1186/s42826-021-00097-x
ISSN
1738-6055
Abstract
Background Particulate matter (PM) is one of the principal causes of human respiratory disabilities resulting from air pollution. Animal models have been applied to discover preventive and therapeutic drugs for lung diseases caused by PM. However, the induced severity of lung injury in animal models using PM varies from study to study due to disparities in the preparation of PM, and the route and number of PM administrations. In this study, we established an in vivo model to evaluate PM-induced lung injury in mice. Results PM dispersion was prepared using SRM2975. Reactive oxygen species were increased in MLE 12 cells exposed to this PM dispersion. In vivo studies were conducted in the PM single challenge model, PM multiple challenge model, and PM challenge with ovalbumin-induced asthma using the PM dispersion. No histopathological changes were observed in lung tissues after a single injection of PM, whereas mild to moderate lung inflammation was obtained in the lungs of mice exposed to PM three times. However, fibrotic changes were barely seen, even though transmission electron microscopy (TEM) studies revealed the presence of PM particles in the alveolar macrophages and alveolar capillaries. In the OVA-PM model, peribronchial inflammation and mucous hypersecretion were more severe in the OVA+PM group than the OVA group. Serum IgE levels tended to increase in OVA+PM group than in OVA group. Conclusions In this study, we established a PM-induced lung injury model to examine the lung damage induced by PM. Based on our results, repeated exposures of PM are necessary to induce lung inflammation by PM alone. PM challenge, in the presence of underlying diseases such as asthma, can also be an appropriate model for studying the health effect of PM.
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