Effect of functional group on activity and stability of lipase immobilized on silica-coated magnetite nanoparticles with different functional group
- Authors
- Lee, Hye Rin; Kim, Moon Il; Hong, Sang Eun; Choi, Jaeyeong; Kim, Young Min; Yoon, Kuk Ro; Lee, Seungho; Ha, Sung Ho
- Issue Date
- Jun-2016
- Publisher
- KOREAN SOC ANALYTICAL SCIENCE
- Keywords
- nanoparticle; silica-coated magnetite nanoparticles; lipase; immobilization; activity; stability
- Citation
- ANALYTICAL SCIENCE AND TECHNOLOGY, v.29, no.3, pp.105 - 113
- Journal Title
- ANALYTICAL SCIENCE AND TECHNOLOGY
- Volume
- 29
- Number
- 3
- Start Page
- 105
- End Page
- 113
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8231
- DOI
- 10.5806/AST.2016.29.3.105
- ISSN
- 1225-0163
- Abstract
- The present study investigated the immobilization of lipases on silica nanoparticles and silica-coated magnetite nanoparticles as supports with a functional group to enhance the stability of lipase. The influence of functional groups, such as the epoxy group and the amine group, on the activity and stability of immobilized lipase was also studied. The epoxy group and the amino group were introduced onto the surface of nanoparticles by glycidyl methacrylate and aminopropyl triethoxysilane, respectively. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles with a functional group showed slightly lower initial enzyme activities than free enzyme; however, the immobilized Candida rugosa lipase retained over 92 % of the initial activity, even after 3 times reuse. Lipase was also immobilized on the silica-coated magnetite nanoparticles by cross-linked enzyme aggregate (CLEA) using glutaraldehyde and covalent binding, respectively, were also studied. Immobilized Candida rugosa lipase on silica nanoparticles and silica-coated magnetite nanoparticles by CLEA and covalent binding showed higher enzyme activities than free enzyme, while immobilized Candida rugosa lipase retained over 73 % of the initial activity after 5 times reuse.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8231)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.