Real-time PCR을 이용한 임플란트주위염 원인균의 정량적 분석Quantitative detection of peri-implantitis bacteria using real-time PCR
- Other Titles
- Quantitative detection of peri-implantitis bacteria using real-time PCR
- Authors
- 김민정; 한경순
- Issue Date
- Oct-2021
- Publisher
- 한국치위생학회
- Keywords
- Multiplex real-time PCR; Oral pathogenic microorganisms; Peri-implantitis; 구강병원성 미생물; 다중 실시간 중합효소 연쇄반응법; 임플란트주위염
- Citation
- 한국치위생학회지, v.21, no.5, pp.555 - 565
- Journal Title
- 한국치위생학회지
- Volume
- 21
- Number
- 5
- Start Page
- 555
- End Page
- 565
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82532
- DOI
- 10.13065/jksdh.20210053
- ISSN
- 2287-1705
- Abstract
- Objectives: This study was conducted to analyze peri-implantitis bacteria and identify their associations with health status and health activities. Methods: Gingival sulcus fluid at the implant’s periodontal pockets sampled from the participants were analyzed by multiplex real time PCR. Results: Participants had strains in the order of 100% F. nucleatum, 98.0% E. corrodens, and 96.0% P. micra, and the correlation between C. rectus and E. nodatum was high (p<0.01). Diabetic group (P. gingivalis, P.
nigrescens) hypertension (P. nigrescens), group with four or more periodontal pockets (P. gingivalis, T. dentica, P. intermedia, E.
nodatum, and C. rectum), smoking (P. micra, E. corrodens), drinking (T. dentola), and scaling groups (C. rectus) were found to have more strains (p<0.05). Conclusions: Representative pathogenic microorganisms detected in periodontal pockets of implants were similar to dental periodontal pockets; however there were differences in the amount and distribution of microorganisms, and they were affected by health status and health behavior.
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