Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification
- Authors
- Kim, Jong-Gil; Baek, Seung Hoon; Kim, Seungrok; Kim, Hae In; Lee, Seung Woo; Le Minh Tu Phan; Kailasa, Suresh Kumar; Park, Tae Jung
- Issue Date
- Dec-2018
- Publisher
- ELSEVIER
- Keywords
- Dengue virus; Specific primers; Aedes aegypi mosquito; Reverse trascription loop-mediated isothermal amplification; Molecular diagnosis
- Citation
- TALANTA, v.190, pp.391 - 396
- Journal Title
- TALANTA
- Volume
- 190
- Start Page
- 391
- End Page
- 396
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/82874
- DOI
- 10.1016/j.talanta.2018.08.019
- ISSN
- 0039-9140
- Abstract
- Dengue virus (DENV) is one of the life-threatening viruses to the human. In this study, we have designed specific novel primers for rapid discriminative detection of DENV-1, DENV-2, and DENV-4 by real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction. The effect of parameters such as reaction temperature and magnesium sulfate was investigated on the RT-LAMP reaction for detection of DENV RNA. Under the optimal conditions, this method is able to differentiate and to detect DENV within 25 min, exhibiting detection limit of 3.5 copies/mu L. Importantly, the novel specific primers-based RT-LAMP assay did not react with other viruses, suggesting the selectivity of the method towards DENV RNA. The RT-LAMP reaction products are easily visualized with naked-eye when irradiated them under UV light at 365 nm. Amplification products could be visualized directly for color changes. This method provides a facile, and accurate molecular amplication technique for the rapid discriminative detection of dengue viruses. The RT-LAMP platform can be used as a promissing diagnostic tool for discriminative detection of DENV without aid of complicated protocols or sophisticated equipment.
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