오가피(Eleutherococcus sessiliflorus)의전연골성 ATDC5 세포의 분화 유도Eleutherococcus sessiliflorus induces differentiation of prechondrogenic ATDC5 Cells
- Other Titles
- Eleutherococcus sessiliflorus induces differentiation of prechondrogenic ATDC5 Cells
- Authors
- 스레스타사로즈쿠마; 송정빈; 이성현; 이동헌; 김호철; 소윤조
- Issue Date
- Jan-2022
- Publisher
- 대한본초학회
- Keywords
- Eleutherococcus sessiliflorus; endochondral bone formation; ATDC5 cells; chondrogenesis; chondrogenic differentiation; BMP-2
- Citation
- 대한본초학회지, v.37, no.1, pp.51 - 59
- Journal Title
- 대한본초학회지
- Volume
- 37
- Number
- 1
- Start Page
- 51
- End Page
- 59
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83414
- ISSN
- 1229-1765
- Abstract
- Objectives : The process through which mesenchymal cells condense and differentiate into chondrocytes to form new bone is known as endochondral bone formation. Chondrogenic differentiation and hypertrophy are essential steps in bone formation and are influenced by various factors. The stem bark and root bark of Eleutherococcus sessiliflorus (ES) have been widely used to treat growth retardation and arthritis in traditional Korean Medicine. In this study, we aimed to investigate the possible role of the stem bark of ES in the stimulation of chondrogenic differentiation in clonal murine chondrogenic ATDC5 cells.
Methods : In ATDC5 cells treated with ES extract, cell viability and extracellular matrix production were determined using CCK-8 assay and Alcian blue staining, respectively, and alkaline phosphatase activity was measured. We also examined mRNA and protein expression levels of genes related to chondrogenic expression in ATDC5 cells using reverse transcription-polymerase chain reaction and western blot analyses.
Results : ES extract increased the accumulation of Alcian blue-stained cartilage nodules and alkaline phosphatase activity in ATDC5 cells. It increased the mRNA expressions of chondrogenic markers including bone sialoprotein (BSP), cartilage collagens, Runt-related transcription factor-2 (RUNX-2), osteocalcin (OCN), β-catenin, and bone morphogenetic protein-2 (BMP-2), as well as the protein expressions of β-catenin, RUNX-2, BMP-2, and alkaline phosphatase (ALP).
Conclusion : Taken together, these results suggest that ES extract exhibits a chondromodulating activity and therefore may be a possible agent for the treatment of bone growth disorders.
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