Cryoprotective effect of turanose on lyophilized Lactobacillus paracasei subsp. paracasei, L. casei 431

Abstract

The lyophilization process is the most convenient and successful method to preserve probiotics, although microorganisms are exposed to conditions of extremely low freezing temperatures as well as dehydration. In this study, we evaluated the cryoprotective effect of turanose on Lactobacillus paracasei subsp. paracasei, L. casei 431 (L. casei 431) as a method to increase survival rate by improving cell viability. The results indicated that the viability of L. casei 431 was 9.6% without the cryoprotective agent, whereas bacterial cell viability was increased to 67.1% with the addition of 12% turanose. When turanose-treated freeze-dried cells were stored at 4 degrees C for 30 days, the survival rate decreased from 67.1 to 53.4%. Furthermore, cell viability significantly decreased by 50% after 30 days when stored at 25 degrees C with the same amount of turanose. Overall, turanose may be used as an effective cryoprotectant to preserve probiotics against the freeze-drying process and for extended storage at 4 degrees C.