Effect of highly branched α-glucans synthesized by dual glycosyltransferases on the glucose release rate
- Authors
- Hong, Moon-Gi; Yoo, Sang-Ho; Lee, Byung-Hoo
- Issue Date
- 15-Feb-2022
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Amylosucrase; Glycogen branching enzyme; Highly branched α-glucans; Slowly digestible property; α-Limit dextrins
- Citation
- Carbohydrate Polymers, v.278
- Journal Title
- Carbohydrate Polymers
- Volume
- 278
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83755
- DOI
- 10.1016/j.carbpol.2021.119016
- ISSN
- 0144-8617
- Abstract
- Increasing α-1,6 linkages in starch molecules generates a large amount of α-limit dextrins (α-LDx) during α-amylolysis, which decelerate the release of glucose at the intestinal α-glucosidase level. This study synthesized highly branched α-glucans from sucrose using Neisseria polysaccharea amylosucrase and Rhodothermus obamensis glycogen branching enzyme to enhance those of slowly digestible property. The synthesized α-glucans (Mw: 1.7–4.9 × 107 g mol−1) were mainly composed of α-1,4 linkages and large proportions of α-1,6 linkages (7.5%–9.9%). After treating the enzymatically synthesized α-glucans with the human α-amylase, the quantity of branched α-LDx (36.2%–46.7%) observed was higher than that for amylopectin (26.8%) and oyster glycogen (29.1%). When the synthetic α-glucans were hydrolyzed by mammalin α-glucosidases, the glucose generation rate decreased because the amount of embedded branched α-LDx increased. Therefore, the macro-sized branched α-glucans with high α-LDx has the potential to be used as slowly digestible material to attenuate postprandial glycemic response. © 2021
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83755)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.