Molecular Weight Determination of Chitosan with Antibacterial Activity Using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry Analysis
- Authors
- 이용현; 박소연; 황유진; 박제권
- Issue Date
- Feb-2022
- Publisher
- 한국고분자학회
- Keywords
- antibacterial effect; chitosan; fine dust; 1; 3-butylene glycol; molecular weight.
- Citation
- Macromolecular Research, v.30, no.2, pp.90 - 98
- Journal Title
- Macromolecular Research
- Volume
- 30
- Number
- 2
- Start Page
- 90
- End Page
- 98
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83818
- DOI
- 10.1007/s13233-022-0013-0
- ISSN
- 1598-5032
- Abstract
- The antibacterial effect of chitosan with different molecular weights was investigated to define their potent biological activity. Both chitosan hydrolysates designated to CTSN-P30 and CTSN-B8 derived from the hydrolysis of high molecular weight chitosan showed significant synergistic antibacterial effects toward Staphylococcus aureus in the presence of 1,3-butylene glycol. The concentration-dependent antimicrobial effect is very important in determining the minimum inhibitory concentration (MIC) of both CTSN-P30 and CTSN-B8. Each MIC50 of CTSN-P30 and CTSNB8 was determined to be about 25 and 50 μg/mL, respectively. Among chitosan hydrolysates, CTSN-P30 can effectively protect the membrane from the penetrating of fine dust which may contain unspecified microorganisms. Also, CTSN-P30, which showed very significant antimicrobial activity, was confirmed to be a hydrolysate containing (GlcNAc)3, and other hydrolysates consisting of (GlcN)5 to (GlcN)15 using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis. These results demonstrated the feasibility of CTSN-P30 and 1,3-butylene glycol to use as a potent antibacterial and find a dustproof agent for the preparation of various industrial products.
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Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
- 보건과학대학 > 의용생체공학과 > 1. Journal Articles
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