Nanoceria-based lateral flow immunoassay for hydrogen peroxide-free colorimetric biosensing for C-reactive protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kong, Do Yun | - |
dc.contributor.author | Heo, Nam Su | - |
dc.contributor.author | Kang, Ji Won | - |
dc.contributor.author | Lee, Jin Bae | - |
dc.contributor.author | Kim, Hae Jin | - |
dc.contributor.author | Kim, Moon Il | - |
dc.date.accessioned | 2022-03-31T03:40:04Z | - |
dc.date.available | 2022-03-31T03:40:04Z | - |
dc.date.created | 2022-01-21 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1618-2642 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83863 | - |
dc.description.abstract | During the recent several decades, lateral flow immunoassay (LFIA) constructed with gold nanoparticle (AuNP) has been widely utilized to conveniently detect target analyte. However, AuNP-based LFIA has limitations, such as limited detection sensitivity and quantification capability. Herein, to overcome these constraints, we have developed cerium oxide nanoparticle (nanoceria)-based LFIA for C-reactive protein (CRP) detection in human serum samples. It was fabricated with nanoceria, a notable nanozyme that shows an oxidase activity to quickly oxidize organic substrate, such as 3,3',5,5'-tetramethylbenzidine (TMB), to produce colored product without any oxidizing agent (e.g., hydrogen peroxide), which is advantageous for realizing point-of-care testing (POCT) applications. By employing human blood serum spiked with CRP, the nanoceria-based LFIA showed two blue-colored lines on the test and control region within 3 min via TMB oxidation, by the captured nanoceria through antigen-antibody interaction. The produced blue-colored lines were distinguished by naked eyes and quantitated with real images acquired by a conventional smartphone with the ImageJ software. With this strategy, target CRP was specifically determined down to 117 ng mL(-1) with high detection precisions yielding coefficient of variation of 9.8-11.3% and recovery of 90.7-103.2% using human blood serum samples. This investigation demonstrates the potential of oxidase-like nanoceria for developing LFIA, which is particularly useful in instrumentation-free POCT environments. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.relation.isPartOf | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.title | Nanoceria-based lateral flow immunoassay for hydrogen peroxide-free colorimetric biosensing for C-reactive protein | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000742594700001 | - |
dc.identifier.doi | 10.1007/s00216-022-03877-z | - |
dc.identifier.bibliographicCitation | ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.414, no.10, pp.3257 - 3265 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85123117259 | - |
dc.citation.endPage | 3265 | - |
dc.citation.startPage | 3257 | - |
dc.citation.title | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | - |
dc.citation.volume | 414 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | Kong, Do Yun | - |
dc.contributor.affiliatedAuthor | Kang, Ji Won | - |
dc.contributor.affiliatedAuthor | Kim, Moon Il | - |
dc.type.docType | Article; Early Access | - |
dc.subject.keywordAuthor | Lateral flow immunoassay (LFIA) | - |
dc.subject.keywordAuthor | Nanoceria | - |
dc.subject.keywordAuthor | Nanozyme | - |
dc.subject.keywordAuthor | Oxidase-like activity | - |
dc.subject.keywordAuthor | C-reactive protein | - |
dc.subject.keywordPlus | SALT-INDUCED AGGREGATION | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | CARDIOVASCULAR RISK | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | NANOZYMES | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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