Nanoceria-based lateral flow immunoassay for hydrogen peroxide-free colorimetric biosensing for C-reactive protein
- Authors
- Kong, Do Yun; Heo, Nam Su; Kang, Ji Won; Lee, Jin Bae; Kim, Hae Jin; Kim, Moon Il
- Issue Date
- Apr-2022
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Lateral flow immunoassay (LFIA); Nanoceria; Nanozyme; Oxidase-like activity; C-reactive protein
- Citation
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.414, no.10, pp.3257 - 3265
- Journal Title
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Volume
- 414
- Number
- 10
- Start Page
- 3257
- End Page
- 3265
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/83863
- DOI
- 10.1007/s00216-022-03877-z
- ISSN
- 1618-2642
- Abstract
- During the recent several decades, lateral flow immunoassay (LFIA) constructed with gold nanoparticle (AuNP) has been widely utilized to conveniently detect target analyte. However, AuNP-based LFIA has limitations, such as limited detection sensitivity and quantification capability. Herein, to overcome these constraints, we have developed cerium oxide nanoparticle (nanoceria)-based LFIA for C-reactive protein (CRP) detection in human serum samples. It was fabricated with nanoceria, a notable nanozyme that shows an oxidase activity to quickly oxidize organic substrate, such as 3,3',5,5'-tetramethylbenzidine (TMB), to produce colored product without any oxidizing agent (e.g., hydrogen peroxide), which is advantageous for realizing point-of-care testing (POCT) applications. By employing human blood serum spiked with CRP, the nanoceria-based LFIA showed two blue-colored lines on the test and control region within 3 min via TMB oxidation, by the captured nanoceria through antigen-antibody interaction. The produced blue-colored lines were distinguished by naked eyes and quantitated with real images acquired by a conventional smartphone with the ImageJ software. With this strategy, target CRP was specifically determined down to 117 ng mL(-1) with high detection precisions yielding coefficient of variation of 9.8-11.3% and recovery of 90.7-103.2% using human blood serum samples. This investigation demonstrates the potential of oxidase-like nanoceria for developing LFIA, which is particularly useful in instrumentation-free POCT environments.
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