Frequencies of glycosylphosphatidylinositol (GPI)-deficient cells using high-sensitivity flow cytometry as per the 2018 ICCS/ESCCA consensus guideline in patients with hematologic malignancy, aplastic anemia, or cytopenia
- Authors
- Kwoun, Woo-Jae; Lee, Hwan Tae; Ahn, Jeong-Yeal
- Issue Date
- Feb-2022
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Paroxysmal nocturnal hemoglobinuria; glycosylphosphatidylinositol deficiency; high-sensitivity flow cytometry; 2018 ICCS; ESCCA; hematologic disorder
- Citation
- EXPERT REVIEW OF HEMATOLOGY, v.15, no.2, pp.175 - 181
- Journal Title
- EXPERT REVIEW OF HEMATOLOGY
- Volume
- 15
- Number
- 2
- Start Page
- 175
- End Page
- 181
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84090
- DOI
- 10.1080/17474086.2022.2038561
- ISSN
- 1747-4086
- Abstract
- Objectives We examined the frequencies and sizes of glycosylphosphatidylinositol (GPI)-deficient cells as per the International Clinical Cytometry Society/European Society for Clinical Cell Analysis (ICCS/ESCCA) consensus guidelines for the high-sensitivity detection of GPI-deficient cells. Methods In 2018, the ICCS/ESCCA guidelines for the high-sensitivity detection of GPI-deficient cells were published. We evaluated frequencies and sizes of GPI-deficient red blood cells (RBCs), neutrophils, and monocytes as determined using the ICCS/ESCCA guidelines and Clinical and Laboratory Standards Institute (CLSI) guidelines in patients with a hematologic malignancy, aplastic anemia, or cytopenia. Results A total of 106 (38.7%) patients exhibited GPI deficiency in at least one blood cell lineage. GPI-deficient cells of one or more lineages were found in 62.7% of patients with a hematologic malignancy, 51.1% of patients with aplastic anemia, and 23.4% of patients with cytopenia. GPI-deficient monocytes were most frequently detected in all three groups. By population size, GPI-deficient clones (>1%) in monocytes were mostly detected in patients with a hematologic malignancy or aplastic anemia. Rare cells with GPI deficiency (<0.1%) in monocytes were most common among patients with cytopenia. Conclusion High-sensitive flow cytometry analysis including monocytes may be necessary for patients with a hematologic disorder.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84090)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.