A Simple, Rapid and Reliable Method to Determine Imipramine and Desipramine in Mouse Serum Using Ultra-High-Performance Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
DC Field | Value | Language |
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dc.contributor.author | Zhao, Jing | - |
dc.contributor.author | Shin, Yujin | - |
dc.contributor.author | Chun, Kwang-Hoon | - |
dc.contributor.author | Yoon, Hye-Ran | - |
dc.contributor.author | Lee, Jeongmi | - |
dc.date.available | 2020-02-28T02:43:31Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 0021-9665 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8447 | - |
dc.description.abstract | A rapid and sensitive ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometric (UHPLC-Q-TOF-MS) method was developed for quantification of imipramine, one of the most widely used tricyclic antidepressants, and desipramine, an active metabolite of imipramine, in mouse serum. The developed method included a simple protein precipitation with acetonitrile in 50 mu L of serum and analyte separation on an Acquity UPLC BEH C18 column using a gradient elution of acetonitrile with 0.1% formic acid and 20 mM ammonium formate. As a result, the entire analysis time was <20 min including the sample preparation and the LC-MS analysis. The limit of quantification was 5.0 ng mL(-1) for both imipramine and desipramine, and calibration curves were linear over the concentration range of 5.0-1,000.0 and 5.0-250.0 ng mL(-1) for imipramine and desipramine, respectively. Intraday precisions at three levels were 2.2-3.6 and 1.7-4.2% for imipramine and desipramine, respectively, whereas interday precisions were 2.6-5.0 and 2.0-8.4% for imipramine and desipramine, respectively. Accuracy ranged between 93.6 and 106.6% for imipramine and 94.1 and 106.4% for desipramine. Absolute recovery was 96.0-97.6% for imipramine and 87.0-99.5% for desipramine. Finally, the described method was applied to mice administered with imipramine, demonstrating the suitability for quantification of imipramine and desipramine for therapeutic drug monitoring or bioequivalence studies. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.relation.isPartOf | JOURNAL OF CHROMATOGRAPHIC SCIENCE | - |
dc.subject | SOLID-PHASE EXTRACTION | - |
dc.subject | UPLC/Q-TOF-MS | - |
dc.subject | HUMAN PLASMA | - |
dc.subject | PROTEIN PRECIPITATION | - |
dc.subject | TRICYCLIC ANTIDEPRESSANTS | - |
dc.subject | QUANTITATIVE BIOANALYSIS | - |
dc.subject | MS/MS METHOD | - |
dc.subject | UV DETECTION | - |
dc.subject | METABOLITES | - |
dc.subject | LC | - |
dc.title | A Simple, Rapid and Reliable Method to Determine Imipramine and Desipramine in Mouse Serum Using Ultra-High-Performance Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000380263900011 | - |
dc.identifier.doi | 10.1093/chromsci/bmv187 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHIC SCIENCE, v.54, no.4, pp.561 - 568 | - |
dc.identifier.scopusid | 2-s2.0-84965177140 | - |
dc.citation.endPage | 568 | - |
dc.citation.startPage | 561 | - |
dc.citation.title | JOURNAL OF CHROMATOGRAPHIC SCIENCE | - |
dc.citation.volume | 54 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Chun, Kwang-Hoon | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SOLID-PHASE EXTRACTION | - |
dc.subject.keywordPlus | UPLC/Q-TOF-MS | - |
dc.subject.keywordPlus | HUMAN PLASMA | - |
dc.subject.keywordPlus | PROTEIN PRECIPITATION | - |
dc.subject.keywordPlus | TRICYCLIC ANTIDEPRESSANTS | - |
dc.subject.keywordPlus | QUANTITATIVE BIOANALYSIS | - |
dc.subject.keywordPlus | MS/MS METHOD | - |
dc.subject.keywordPlus | UV DETECTION | - |
dc.subject.keywordPlus | METABOLITES | - |
dc.subject.keywordPlus | LC | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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