A Simple, Rapid and Reliable Method to Determine Imipramine and Desipramine in Mouse Serum Using Ultra-High-Performance Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
- Authors
- Zhao, Jing; Shin, Yujin; Chun, Kwang-Hoon; Yoon, Hye-Ran; Lee, Jeongmi
- Issue Date
- Apr-2016
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- JOURNAL OF CHROMATOGRAPHIC SCIENCE, v.54, no.4, pp.561 - 568
- Journal Title
- JOURNAL OF CHROMATOGRAPHIC SCIENCE
- Volume
- 54
- Number
- 4
- Start Page
- 561
- End Page
- 568
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/8447
- DOI
- 10.1093/chromsci/bmv187
- ISSN
- 0021-9665
- Abstract
- A rapid and sensitive ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometric (UHPLC-Q-TOF-MS) method was developed for quantification of imipramine, one of the most widely used tricyclic antidepressants, and desipramine, an active metabolite of imipramine, in mouse serum. The developed method included a simple protein precipitation with acetonitrile in 50 mu L of serum and analyte separation on an Acquity UPLC BEH C18 column using a gradient elution of acetonitrile with 0.1% formic acid and 20 mM ammonium formate. As a result, the entire analysis time was <20 min including the sample preparation and the LC-MS analysis. The limit of quantification was 5.0 ng mL(-1) for both imipramine and desipramine, and calibration curves were linear over the concentration range of 5.0-1,000.0 and 5.0-250.0 ng mL(-1) for imipramine and desipramine, respectively. Intraday precisions at three levels were 2.2-3.6 and 1.7-4.2% for imipramine and desipramine, respectively, whereas interday precisions were 2.6-5.0 and 2.0-8.4% for imipramine and desipramine, respectively. Accuracy ranged between 93.6 and 106.6% for imipramine and 94.1 and 106.4% for desipramine. Absolute recovery was 96.0-97.6% for imipramine and 87.0-99.5% for desipramine. Finally, the described method was applied to mice administered with imipramine, demonstrating the suitability for quantification of imipramine and desipramine for therapeutic drug monitoring or bioequivalence studies.
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