Rational Development of Co-Doped Mesoporous Ceria with High Peroxidase-Mimicking Activity at Neutral pH for Paper-Based Colorimetric Detection of Multiple Biomarkers
- Authors
- Nguyen, Phuong Thy; Lee, Junsang; Cho, Ara; Kim, Min Su; Choi, Daeeun; Han, Jeong Woo; Kim, Moon Il; Lee, Jinwoo
- Issue Date
- May-2022
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- biosensing; density functional theory; mesoporous ceria; neutral pH peroxidase-like nanozymes; paper-based microfluidic devices
- Citation
- Advanced Functional Materials, v.32, no.21
- Journal Title
- Advanced Functional Materials
- Volume
- 32
- Number
- 21
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84518
- DOI
- 10.1002/adfm.202112428
- ISSN
- 1616-301X
- Abstract
- Peroxidase-mimicking nanozymes have been extensively studied, however, their application is limited by the requirement for an acidic pH. Herein, the development of Co-doped mesoporous cerium oxide (Co-m-ceria) is reported, which operates optimally at a near-neutral pH and exhibits a peroxidase-like catalytic efficiency that is 600-times higher than that of pristine m-ceria. Density functional theory (DFT) calculations for the application of pristine and various metal-doped m-ceria in peroxidase-like reactions under different pH environments are conducted to select Co as the appropriate dopant. The high peroxidase-like activity of Co-m-ceria under neutral conditions and its mesoporous nature enable its application in a one-pot cascade reaction system, wherein biomarkers of oxidative enzymes can be detected without altering the pH. Five different oxidative enzymes are immobilized in the pores of Co-m-ceria at high loadings, followed by incorporation of the enzyme-containing Co-m-ceria in paper microfluidic devices for the convenient and simultaneous detection of multiple biomarkers. The Co-m-ceria-incorporated paper microfluidic device enables the selective and sensitive determination of multiple biomarkers using a smartphone-acquired image. This study demonstrates the potential of the rational design of nanozymes and their application in paper microfluidic devices, laying the groundwork for future applications of nanozymes in point-of-care testing environments.
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