Estrogenic activity of freeze-dried silkworm extracts through the activation of estrogen receptors in MCF-7 cellsopen access
- Authors
- Lee, Sullim; Nguyen, Quynh Nhu; Kim, Sung Jin; Lee, Joohwan; Shin, Myoung-Sook
- Issue Date
- Dec-2022
- Publisher
- SPRINGER SINGAPORE PTE LTD
- Keywords
- Silkworm; 1-Deoxynojirimycin; MCF-7 breast cancer cells; Estrogen
- Citation
- Applied Biological Chemistry, v.65, no.1, pp.1 - 9
- Journal Title
- Applied Biological Chemistry
- Volume
- 65
- Number
- 1
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84876
- DOI
- 10.1186/s13765-022-00714-w
- ISSN
- 2468-0834
- Abstract
- Silkworm is an insect that feeds on mulberry leaves only, and silkworm powder has been reported to have antioxidant, hypoglycemic, and anticholesterol activities. In this study, we measured the content of 1-deoxynojirimycin (1-DNJ) in 24 different extracts from silkworm cocoons and also investigated the estrogenic activities of these extracts and their effects on the activation of intracellular signaling pathways in MCF-7 breast cancer cells. Among the 24 silkworm extracts, relatively high 1-DNJ content and estrogenic activity were shown by Extract 11 (30E3H20-1), which was prepared by a single extraction of silkworm powder with 20 times the volume of 30% ethanol against each powder weight for 3 h. The estrogen receptor activity of this extract was confirmed based on its promoting effects on the phosphorylation of the estrogen receptor α (ERα) and mRNA expression of the
ESR1
and
ESR2
genes. In addition, treatment with Extract 11 (30E3H20-1) increased the phosphorylation of AKT, p38, and JNK, which are downstream proteins of ERα. Based on our findings, a silkworm extract could be developed as a natural estrogen supplement in the future.
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Collections - 한의과대학 > 한의예과 > 1. Journal Articles
- 바이오나노대학 > 생명과학과 > 1. Journal Articles
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