Prediction and identification of new type holin protein of Escherichia coli phage ECP26
DC Field | Value | Language |
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dc.contributor.author | Park, Do-Won | - |
dc.contributor.author | Lee, Jong Hun | - |
dc.contributor.author | Park, Jong-Hyun | - |
dc.date.accessioned | 2022-07-19T02:40:28Z | - |
dc.date.available | 2022-07-19T02:40:28Z | - |
dc.date.created | 2022-07-19 | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 1226-7708 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84996 | - |
dc.description.abstract | Bacteriophages (phages) infecting specifically target bacteria utilize a unique lysis module known as the holin-endolysin cassette to release progeny. Studies on the phage lytic proteins could contribute to the development of alternatives to antibiotics. Here, we predicted and identified the holin protein of rV5-like phage ECP26 for increasing lytic activity of the phage endolysin. In silico analysis revealed that open reading frame 151 (ORF151) of ECP26 contained two transmembrane domains. Co-expression of endolysin with ORF151 resulted in the cell lysis of Escherichia coli, suggesting that ORF151 protein functioned as the holin that disrupted the cytoplasmic membrane. The putative holin showed a high amino acid homology by more than 80% to the predicted holins of rV5-like phages. Therefore, the holin protein would be helpful for developing efficient lysis strategies with endolysin against gram-negative E. coli. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST | - |
dc.relation.isPartOf | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.title | Prediction and identification of new type holin protein of Escherichia coli phage ECP26 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000806649300001 | - |
dc.identifier.doi | 10.1007/s10068-022-01089-w | - |
dc.identifier.bibliographicCitation | FOOD SCIENCE AND BIOTECHNOLOGY, v.31, no.7, pp.843 - 847 | - |
dc.identifier.kciid | ART002859998 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85131568196 | - |
dc.citation.endPage | 847 | - |
dc.citation.startPage | 843 | - |
dc.citation.title | FOOD SCIENCE AND BIOTECHNOLOGY | - |
dc.citation.volume | 31 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Park, Do-Won | - |
dc.contributor.affiliatedAuthor | Lee, Jong Hun | - |
dc.contributor.affiliatedAuthor | Park, Jong-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | Holin | - |
dc.subject.keywordAuthor | rV5-like phage | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordPlus | BACTERIOPHAGE | - |
dc.subject.keywordPlus | LACTOCOCCUS | - |
dc.subject.keywordPlus | ENDOLYSINS | - |
dc.subject.keywordPlus | BARRIERS | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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