Prediction and identification of new type holin protein of Escherichia coli phage ECP26
- Authors
- Park, Do-Won; Lee, Jong Hun; Park, Jong-Hyun
- Issue Date
- Jul-2022
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- Bacteriophage; Holin; rV5-like phage; Escherichia coli
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.31, no.7, pp.843 - 847
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 31
- Number
- 7
- Start Page
- 843
- End Page
- 847
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/84996
- DOI
- 10.1007/s10068-022-01089-w
- ISSN
- 1226-7708
- Abstract
- Bacteriophages (phages) infecting specifically target bacteria utilize a unique lysis module known as the holin-endolysin cassette to release progeny. Studies on the phage lytic proteins could contribute to the development of alternatives to antibiotics. Here, we predicted and identified the holin protein of rV5-like phage ECP26 for increasing lytic activity of the phage endolysin. In silico analysis revealed that open reading frame 151 (ORF151) of ECP26 contained two transmembrane domains. Co-expression of endolysin with ORF151 resulted in the cell lysis of Escherichia coli, suggesting that ORF151 protein functioned as the holin that disrupted the cytoplasmic membrane. The putative holin showed a high amino acid homology by more than 80% to the predicted holins of rV5-like phages. Therefore, the holin protein would be helpful for developing efficient lysis strategies with endolysin against gram-negative E. coli.
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