Shotgun proteomics of extracellular matrix in late senescent human dermal fibroblasts reveals a down-regulated fibronectin-centered networkopen access
- Authors
- Cho, Kun; Yang, Kyeong Eun; Nam, Soo-Bin; Lee, Song-I.; Yeo, Eui-Ju; Choi, Jong-Soon
- Issue Date
- Jun-2022
- Publisher
- SPRINGER INT PUBL AG
- Keywords
- Extracellular matrix; Human dermal fibroblasts; Late senescence; Shotgun proteomics; Protein network analysis
- Citation
- JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, v.13, no.1
- Journal Title
- JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
- Volume
- 13
- Number
- 1
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85002
- DOI
- 10.1186/s40543-022-00329-2
- ISSN
- 2093-3134
- Abstract
- Extracellular matrix (ECM) proteins play a pivotal role in cell growth and differentiation. To characterize aged ECM proteins, we compared the proteomes by shotgun method of young (passage #15) and late senescent (passage #40) human dermal fibroblasts (HDFs) using SDS-PAGE coupled with LC-MS/MS. The relative abundance of identified proteins was determined using mol% of individual proteins as a semi-quantitative index. Fifteen ECM proteins including apolipoprotein B (APOB) and high-temperature requirement factor 1 (HTRA1) were up-regulated, whereas 50 proteins including fibronectin 1 (FN1) and vitronectin (VTN) were down-regulated in late senescent HDFs. The identified ECM proteins combined with plasma membrane were queried to construct the protein-protein interaction network using Ingenuity Pathways Analysis, resulting in a distinct FN1-centered network. Of differentially abundant ECM proteins in shotgun proteomics, the protein levels of FN1, VTN, APOB, and HTRA1 were verified by immunoblot analysis. The results suggest that the aging process in HDFs might be finally involved in the impaired FN1 regulatory ECM network combined with altered interaction of neighboring proteins. Shotgun proteomics of highly aged HDFs provides insight for further studies of late senescence-related alterations in ECM proteins.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/85002)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.